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Figure 5. Confocal microscopy analysis of NOS2 expression in BMMC cultured with different conditions. NOS2 expression in BMMCIL-3 and BMMCSCF/IL-4 after IFN- treatment for 24 h was examined with confocal microscopy analysis using <t>anti-CD117</t> and anti-NOS2 antibody. Antibody la- beled with goat anti-mouse CD117 and rabbit anti-mouse NOS2 was detected with AlexaFluor 546-conjugated donkey anti-goat IgG (green) and AlexaFluor 647-conjugated donkey anti-rabbit IgG (red), respectively. Nucleus of cells was stained with DAPI (blue). Results are representative of four sepa- rate experiments with four different BMMC cultures from different mice.
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Figure 5. Confocal microscopy analysis of NOS2 expression in BMMC cultured with different conditions. NOS2 expression in BMMCIL-3 and BMMCSCF/IL-4 after IFN- treatment for 24 h was examined with confocal microscopy analysis using <t>anti-CD117</t> and anti-NOS2 antibody. Antibody la- beled with goat anti-mouse CD117 and rabbit anti-mouse NOS2 was detected with AlexaFluor 546-conjugated donkey anti-goat IgG (green) and AlexaFluor 647-conjugated donkey anti-rabbit IgG (red), respectively. Nucleus of cells was stained with DAPI (blue). Results are representative of four sepa- rate experiments with four different BMMC cultures from different mice.
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Figure 5. Confocal microscopy analysis of NOS2 expression in BMMC cultured with different conditions. NOS2 expression in BMMCIL-3 and BMMCSCF/IL-4 after IFN- treatment for 24 h was examined with confocal microscopy analysis using <t>anti-CD117</t> and anti-NOS2 antibody. Antibody la- beled with goat anti-mouse CD117 and rabbit anti-mouse NOS2 was detected with AlexaFluor 546-conjugated donkey anti-goat IgG (green) and AlexaFluor 647-conjugated donkey anti-rabbit IgG (red), respectively. Nucleus of cells was stained with DAPI (blue). Results are representative of four sepa- rate experiments with four different BMMC cultures from different mice.
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Figure 5. Confocal microscopy analysis of NOS2 expression in BMMC cultured with different conditions. NOS2 expression in BMMCIL-3 and BMMCSCF/IL-4 after IFN- treatment for 24 h was examined with confocal microscopy analysis using <t>anti-CD117</t> and anti-NOS2 antibody. Antibody la- beled with goat anti-mouse CD117 and rabbit anti-mouse NOS2 was detected with AlexaFluor 546-conjugated donkey anti-goat IgG (green) and AlexaFluor 647-conjugated donkey anti-rabbit IgG (red), respectively. Nucleus of cells was stained with DAPI (blue). Results are representative of four sepa- rate experiments with four different BMMC cultures from different mice.
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Image Search Results


Antibodies

Journal: The Journal of Physiology

Article Title: Comparison of inhibitory neuromuscular transmission in the Cynomolgus monkey IAS and rectum: special emphasis on differences in purinergic transmission

doi: 10.1113/JP275437

Figure Lengend Snippet: Antibodies

Article Snippet: A list of the primary antibodies is given in Table . table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibody Source Host Working dilution Anti‐human PDGFRα R&D Systems, Minneapolis, MN, USA Goat 1:100 Anti‐human SCFR (Kit) R&D Systems Goat 1:100 Anti‐nNOS (used with anti‐Kit and anti‐PDGFRα) Santa Cruz Biotechnology Inc., Dallas, TX, USA Rabbit 1:500 Anti‐nNOS (used with anti‐VIP) Gift from Dr Piers Emson, Molecular Science Group, Cambridge, UK Sheep 1:500 Anti‐SK3 Alomone Labs, Jerusalem, Israel Rabbit 1:500 Anti‐VIP Immunostar Antibodies, Hudson, WI, USA Rabbit 1:500 Open in a separate window Antibodies Imaging Cryosections were examined using a Zeiss LSM 510 confocal microscope (Carl Zeiss, Thornwood, NY, USA).

Techniques:

Figure 5. Confocal microscopy analysis of NOS2 expression in BMMC cultured with different conditions. NOS2 expression in BMMCIL-3 and BMMCSCF/IL-4 after IFN- treatment for 24 h was examined with confocal microscopy analysis using anti-CD117 and anti-NOS2 antibody. Antibody la- beled with goat anti-mouse CD117 and rabbit anti-mouse NOS2 was detected with AlexaFluor 546-conjugated donkey anti-goat IgG (green) and AlexaFluor 647-conjugated donkey anti-rabbit IgG (red), respectively. Nucleus of cells was stained with DAPI (blue). Results are representative of four sepa- rate experiments with four different BMMC cultures from different mice.

Journal: Journal of leukocyte biology

Article Title: Microenvironmental regulation of inducible nitric oxide synthase expression and nitric oxide production in mouse bone marrow-derived mast cells.

doi: 10.1189/jlb.0811436

Figure Lengend Snippet: Figure 5. Confocal microscopy analysis of NOS2 expression in BMMC cultured with different conditions. NOS2 expression in BMMCIL-3 and BMMCSCF/IL-4 after IFN- treatment for 24 h was examined with confocal microscopy analysis using anti-CD117 and anti-NOS2 antibody. Antibody la- beled with goat anti-mouse CD117 and rabbit anti-mouse NOS2 was detected with AlexaFluor 546-conjugated donkey anti-goat IgG (green) and AlexaFluor 647-conjugated donkey anti-rabbit IgG (red), respectively. Nucleus of cells was stained with DAPI (blue). Results are representative of four sepa- rate experiments with four different BMMC cultures from different mice.

Article Snippet: Goat anti-mouse CD117 polyclonal antibody was obtained from R&D Systems (Minneapolis, MN, USA).

Techniques: Confocal Microscopy, Expressing, Cell Culture, Staining